EasyDiver+
EasyDiver+ is an improved build of the EasyDIVER tool. While EasyDIVER was a pipeline exclusively to process paired-end sequencing data, EasyDiver+ can process, analyze and visualize sequencing data from consecutive rounds of in vitro evolution.
The main improvements in EasyDiver+ compared to the original EasyDIVER are:
Straightforward, user-friendly GUI.
Option to select from multiple genetic code dictionaries for translation.
Option to analyze sequencing data, calculating metrics for the relative enrichment across consecutive rounds of selection.
Unique sequence names consistently used across rounds of selection to faciliate visual identification of variants of interest.
Estimation of 95% confidence intervals for sequence counts.
Graphical outputs with user defined cutoff values.